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nanoKAPsm 2008

nano: Knowledge • Application • Production
Utilizing Nanotechnology for Detection of Toxins and Pathogens




Wednesday, November 12, 2008 

9:00  Registration, Exhibit Viewing/Poster Setup, Coffee and Pastries 
10:10 Organizer’s Welcome and Opening Remarks
10:15 Nanoparticles Based Biosensors for Sensitive Detection of Biomarkers 
Yuehe Lin, PhD, Laboratory Fellow, Pacific Northwest National Laboratory 
The utilization of functional nanoparticles for biosensing has generated a great deal of interest because they are found to greatly enhance sensitivity of various given methods. Electrochemical biosensors based on nanoparticle labels are very attractive for bioassays, due to their high sensitivity, inherent simplicity, miniaturization, and low cost. In this presentation, we will give an overview on recent development of nanoparticles based biosensors for selective and sensitive detection of protein biomarkers.
10:45 Nanoparticles and Quantum Dots for Biodiagnostic Applications 
Arnold Kell, PhD, and Benoit Simard, PhD, Principal Research Officer, Steacie Institute for Molecular Sciences, National Research Council, Canada*
Infectious and chronic diseases and bioterrorism are of tremendous global concern. The National Research Council of Canada is dedicated to developing nanoparticle platforms to aid in the rapid detection of these deadly diseases and security threats. Specifically, this presentation will highlight our efforts to utilize unique surface ligands anchored to magnetic nanoparticles, dye-doped silica nanoparticles and quantum dots to enable the selective labeling, magnetic confinement and optical detection of a variety of potentially harmful bacteria and cells in biological samples.
*In collaboration with: Kui Yu 
11:15 DNA-Tile-Array Based Biosensors 
Yan Liu, PhD, Assistant Professor, Dept of Chemistry and Biochemistry, Biodesign Institute, Center of Single Molecule Biophysics, Arizona State University 
We describe a new concept using combinatorial self-assembly of DNA nanotiles into micrometer-sized two-dimensional arrays that carry nucleic acid probes and barcoded fluorescent dyes to achieve multiplexed detection. The specificity and sensitivity of the arrays by detecting multiple DNA sequences and aptamer binding molecules were demonstrated. Accurate control of the interprobe distances and solution-based binding reactions ensures fast target binding kinetics. The detection sensitivity can be future improved using hybridization chain reaction (HCR) as a signal amplifier.
11:45 Networking Refreshment Break, Exhibit/Poster Viewing 
12:15 Electric Field Assisted Nano-Assembly and Detection on Microarrays 
Dalibor Hodko, PhD, Director, Advanced Technology, Nanogen, Inc. 
Principles and examples of electronic microarray applications for electric-field assisted (nano-)assembly of molecules and components for sensors and detection of pathogens will be summarized. Electric field and hydrodinamically enhanced biochemical reactions will be demonstrated in the array format such as rolling circle amplification for isothermal on-chip amplification with sensitivity approaching few molecules. Attempts toward miniaturization of the electronic microarray platform will be summarized with intended point-of-care applications.
12:45 Point-Of-Care Detection of Staphylococcal Enterotoxins 
Hugh Bruck, PhD, Associate Professor of Mechanical Engineering, University of Maryland; and 
Avi Rasooly, PhD, Program Director, Cancer Diagnosis Program, National Cancer Institute 
Staphylococcal enterotoxins (SEs) are amajor cause of food-borne diseases. Traditionally, SEs assayed immunologically with ELISA. Carbon nanotubes’ (CNT) uniquemechanical and electronic properties combined with a large specific surface areamake them attractive for biosensing.We applied CNT to increase the sensitivity of a simple and portable point-of-care immunosensor based on the detection of Enhanced chemiluminescence (ECL) by a cooled Charge- Coupled Device (CCD) detector. This combination of ECL, CNT and CCD technologies is used to improve the detection of Staphylococcal Enterotoxin B (SEB) in food. Anti-SEB primary antibodies were immobilized onto the CNT surface and the antibody-nanotube mixture was immobilized onto a polycarbonate surface. SEB was then detected by a sandwich-type ELISA assay on the CNTpolycarbonate surface with an ECL assay. SEB in buffer, soymilk, apple juice, andmeat baby food was assayed with a limit of SEB with CCD detector is 0.01 ng/mL similar to the detection limit of a fluorometric detector, which is farmore sensitive than the conventional ELISA detection limit of ~1 ng/ml. Our simple, versatile and inexpensive CCD based Point-of-care detector combined with the CNT-ECL immunosensormethod can be used to simplify and increase sensitivity formany other types of diagnostics and detection assays.
1:15 Lunch 
2:30  Simultaneous Multiplexed Single-Molecule Detection of DNA, RNA, and Proteins 
Michael D. Berg, PhD, Chief Scientific Officer, Attometrics, Inc. 
We report the construction of a novel biosensing nanodevice to detect single molecules of target DNA, RNA, or protein on the same platform at the same time. The combination of reliable specificity, high sensitivity, and low cost should make this technology a viable option for widespread clinical use. Early diagnosis of diseases such as cancer can have a profound impact on a patient’s prognosis. The diagnoses are dependent on rapid, sensitive detection of specific protein and/or nucleic acid biomarkers. Rapid, sensitive detection and identification of pathogens is also critically important to minimize the transfer and spread of disease as well as to devise and evaluate effective treatment strategies.
3:00  TessArray® Resequencing Pathogen Microarray (RPM) is a Reliable Shannon Network Comprised of Less Reliable Nanoscale Information Transducers (PCR-Like Primer Probes) 
Clark Tibbetts, PhD, Executive Vice President and CTO, Tessarae, LLC*
Oligonucleotide probes that are used to amplify target gene sequences are nanoscale information transducers. A single 25-nucleotide probe (8 nm length) represents extraordinary sequence specificity, as only one of 1015 different sequences of the four bases A, G, C and T. Such probes are noisy channels, due to idiosyncratic interactions of similar gene sequences from distinct organisms as well as specific mutations within a species. This accounts for unacceptably high rates of false negative and false positive results from PCR-based tests in critical applications. Claude Shannon and Edward Moore demonstrated that reliable (error-free) communications circuits result from networks comprised of less reliable (“crummy”) components or relays. This principle underlies the superior sensitivity, specificity and multiplicity of the TessArray Resequencing Pathogen Microarray (RPM) for simultaneous detection and identification many different viruses and bacteria in a single specimen. An RPM detector array network of almost one million different oligonucleotide probes detects and specifies almost 125,000 bases of virtually error-free pathogen gene nucleotide sequences. A real-world TessArray RPM application example illustrates rapid and unequivocally accurate detection and identification of any HN subtype of human and/or avian influenza virus.
*In collaboration with: K.Schafer and M.Lorence, TessArae; D.Swayne and Colleen Thomas, U.S. Dept of Agriculture 
3:30  Detection and Subtyping of Avian Influenza Virus and Foot-and-Mouth Disease Virus Using the NanoChip400 Electronic Microarray 
Oliver Lung, PhD, Research Scientist, Canadian Food Inspection Agency, Animal Diseases Research Institute, Canada*
Avian influenza virus (AIV) and Foot-and-Mouth Disease virus (FMDV) outbreaks can result in severe economic losses. We developed two electronic microarrays that can subtype all 16 H (hemagglutinin) subtypes of AIV, and all 7 serotypes of FMDV. Probes were also designed against the conserved regions of the AIV matrix (M) gene and FMDV polymerase coding region to detect all AIV and FMDV, respectively. Thirty-two influenza isolates representing all 16 H subtypes, and 23 FMDV isolates representing all 7 serotypes were correctly identified and typed using the two arrays.
*In collaboration with: A.Beeston, J.Geddes, K.Burton Hughes, J.Pasick, A.Clavijo, T.Furukawa-Stoffer, W.Mauro, and D.Deregt, Canadian Food Inspection Agency; and D.Hodko, Nanogen
4:00  Networking Refreshment Break, Exhibit/Poster Viewing 
4:30  Chemical and Biosensor Applications Based on Hybrid Nanojunction Arrays 
NJ Tao, PhD, Professor of Electrical Engineering; and 
Erica Forzani, PhD, Research Assistant Professor, Chem- & Bio- Sensors, Center for Bioelectronics and Biosensors, Biodesign Institute, Arizona State University 
Abstract not available at time of printing. Please visit for the latest Program updates.
5:00  Nanotoxicology: From Emergency Services Response to Environmental Health Considerations 
Michelle Cadieux, MBA, HM FRO, CNCP, Researcher, Disaster Services Professional, NanEngineering (NASA)/ Community Safety Programs 
Chemical detection equipment is becoming more and more sensitive as the particles are smaller, yet not all chemical sampling equipment is in this range yet. Nano sized particles have different properties than the regular chemicals. There are already studies finding carbon nanotubes have asbestos like qualities and the bioaccumulative effects found in fish brains. Come discuss nanotoxicology, how the industry is changing. Resources on PPE, which MSDS chemical databases track at this level, and a survey of field sampling equipment that work on the nano scale. The Nano Safety Consortium is working with the EPA to have companies voluntarily come forward with safety data.
5:30  Concluding Discussion, End of Symposium 

Industry and academic scientists are encouraged to submit poster titles for this event. One-page abstracts (8 1/2" x 11" with 1-inch margins) must be submitted via e-mail no later than October 20, 2008 for inclusion in conference documentation. Additional poster submissions will be accepted until November 5, 2008 but may not be included in conference documentation.

DIMENSIONS of the poster boards are: 
4 feet wide by 3 feet high 
(although posterboards could be placed vertically as well and then the dimentsions obviously would be 3' w x 4' h, or 90 x 120cm accordingly).

Note: If you're submitting a poster, you MUST be registered and paid registration fee plus posterboard reservation fee in advance to ensure that a posterboard is reserved for you.


Registration fee includes lunch on the first day of the Main Conference, refreshments, access to posters and exhibit, and all documentation made available to us by speakers.

Become a member of the Knowledge Foundation Technology Commercialization Alliance and save NOW 15% of your conference registration or publications purchase. 
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* On-site registration - add US $100 to below amounts 

- Commercial Registration for Detection Technologies PLUS NanoKap Symposium 

Non-member: US $1599.00 
Member: US $1359.00 

- Commercial Registration for Detection Technologies ONLY 

Non-member: US $1199.00 
Member: US $1019.00 

- Academic/Government Registration for Detection Technologies PLUS NanoKap Symposium: 

Non-member: US $1099.00 
Member: US $934.00

- Academic/Government Registration for Detection Technologies ONLY: 

Non-member: US $799.00 
Member: US $679.00

- Poster Space Reservation fee: US $65 (you must be registered for the Conference) 

The academic/government rate is extended to all participants registering as full time employees of government and universities. To receive the academic/government rate you must not be affiliated with any private organizations either as consultants or owners or part owners of businesses.

All payments must be made in U.S. funds drawn on a U.S. bank. Please make check(s) payable to The Knowledge Foundation and attach to the registration form even if you have registered by phone, fax or e-mail. To guarantee your registration, payment must be received prior to the conference. Confirmation of your booking will follow.

Exhibiting/Sponsoring Please contact Lindsay Kennedy at (617) 232-7400 ext. 210 or email for all inquiries.

Discount Accommodations and Travel
A block of rooms has been allocated at a special reduced rate. Please make your reservations by October  14, 2008 to obtain this rate. When making reservations, please refer to The Knowledge Foundation. Contact The Knowledge Foundation if you require assistance.

Sheraton Crescent Hotel
2620 West Dunlap 
Phoenix, AZ 85021

For Hotel Reservations Contact
Andersen Travel at 
Tel: (508) 429-6494 or 1-800-229-6494 
Fax: (508) 429-7380 

The Knowledge Foundation's official travel agent, Andersen Travel will assist you in making all or a portion of your travel arrangements.

A substitute member of your company may replace your attendance at any time at no charge if you find your schedule prevents you from attending. Please notify us immediately so that materials can be prepared. If you do not wish to substitute your registration, we regret that your cancellation will be subject to a $100 processing fee. To receive a prompt refund, we must receive your cancellation in writing 30 days prior to the conference. Unfortunately cancellations cannot be accepted after that date. In the event that The Knowledge Foundation cancels an event, The Knowledge Foundation cannot resume responsibility for any travel-related costs.