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  Biodetection Agenda   
  Sponsoring Opportunities   
  Food Safety Agenda   
  Biosurveillance Agenda   

Traditional means for microbial detection can no longer match the pace of today’s food processing and global distribution networks. At the same time, rapid detection of pathogens in foods has never been more important. In the U.S. alone, there are an estimated 47.8 million illnesses, over 127,000 hospitalizations and 3,000-plus deaths attributed to foodborne illness each year. Emerging sensor and detection platforms can provide the timely and actionable information needed to lessen the human and economic burdens levied by foodborne disease. This symposium features presentations on emerging optical, nanotechnological, spectroscopic and electrochemical technologies for pathogen detection, including label-free and high-throughput methods. Novel ligands for pathogen capture will also be examined. Benefits and challenges of these new methods and their comparison with existing techniques will be discussed. Label-free approaches and the advantages of novel bioaffinity ligands will be highlighted.
Talks by leading academics and researchers in the military and private sectors will include:

•    Light scattering and spectral pattern recognition for bacterial detection
•    Flow cytometry for rapid detection of pathogens in complex matrices
•    Bioluminescent bacteriorphage for high-throughput detection of pathogens 
•    Antimicrobial peptides as bioaffinity ligands for pathogen detection
•    Parallel capillary electrophoresis for high-throughput detection and characterization of pathogens

Thursday, June 12, 2014 
2:00  Registration with Exhibit & Poster Viewing 
2:20 Chairperson’s Welcome and Opening Remarks
Byron Brehm-Stecher, Ph.D., Associate Professor, Iowa State University 

2:30  Light Scattering and Optical Sensors for High Throughput Screening of Bacterial Pathogens
Arun Bhunia, BVSc, Ph.D., Professor of Molecular Food Microbiology, Center for Food Safety Engineering, Dept. of Food Science and Dept. of Comparative Pathobiology, Purdue University

Rapid high throughput pathogen screening tools are crucial in improving safety of our food supply. Current pathogen detection trend emphasizes the application of single platform for detection of multiple pathogens/toxins in a cost effective manner. Our research team is currently developing high throughput nano/biosensor-based screening tools such as fiber optic, cell-based and light scattering for detection of multi-pathogens and toxins from food. Cell-based sensor determines virulence potential and is suitable for confirmation of pathogens or toxins present in a sample while light scattering sensor is a label-free method for real-time detection and identification of bacterial colonies on agar plates. While light scattering sensor can detect and identify bacterial colonies on plate without the use of any labeling reagents. It can also be used for bacterial community analysis on products and for process verification, and hygiene monitoring. Limit of detection, specificity, automation and application of each sensor with various sample matrices will be discussed. 
3:00  Flow Cytometry for Rapid Detection of Pathogens in Complex Matrices
Byron Brehm-Stecher, Ph.D., Associate Professor, Iowa State University
Flow cytometry (FCM) is a rapid method for analysis of cells in liquid suspension. Although FCM was originally developed for measurement of mammalian cells, it has found increasing use in microbiology over the past two decades. FCM allows collection of data at the single cell level on cell physiology, number, viability, genetic identity and other parameters. Depending on the staining technique used, FCM can also be a very robust means for analysis of complex samples, enabling operators to differentiate between cells of interest and high backgrounds of particulate matter or non-target microflora. This talk will provide an overview of FCM and its applications in food microbiology for the detection and characterization of foodborne microorganisms. 
3:30  Parallel Capillary Electrophoresis for High-Throughput Detection and Characterization of Pathogens
Pierre Vareneau, Ph.D., Chief Technology Officer, Advanced Analytical Technologies, Inc.
Capillary electrophoresis (CE) has long been a workhorse technology in analytical chemistry labs. CE provides rapid and efficient separations of biologically relevant molecules or compounds, can be used to analyze small sample volumes and typically requires minimal sample preparation. A key advantage of CE is its superior ability to separate complex pools of nucleic acid fragments that present distinct challenges to the gel-based electrophoresis systems that are most commonly used for DNA analysis. This talk will focus on the use of high-throughput parallel CE for nucleic acid-based analysis of foodborne pathogens, contrasting this approach from traditional analyses, including gel-based systems. Additional applications of parallel CE, including next-generation sequencing and related uses will be discussed. 
4:00  Refreshments with Exhibit & Poster Viewing 

4:30  Antimicrobial Peptide Sensor Arrays for Endotoxin Detection and Discrimination: Towards Microbial Forensics Applications
Joshua R. Uzarski, PhD, Research Scientist, Battelle Memorial Institute, contractor for US Army Natick Soldier Research, Development, and Engineering Center

Lipopolysaccharides, also called endotoxin, shed from Gram negative bacterial pathogens, are the cause of septic shock syndrome and are a pressing issue in sterile environments including health and food preparation facilities. Current detection methods are insufficient, being both temporally and monetarily expensive. We have developed a method using a small array of antimicrobial peptides that detect and discriminate LPS molecules from different bacterial species, strains, and also from different original growth conditions. The latter example might help identify the source of an endotoxin or pathogen outbreak, augmenting the field of microbial forensics. 

5:00  Bioluminescence-transducing phage for detection/identification of E. coli O157:H7 
Bruce Applegate, Associate Professor in Biological Sciences and Food Science, Purdue University*

Shiga-toxigenic strains of E. coli, especially E. coli O157:H7, are most frequently associated with beef and produce, and they can cause serious disease such as severe bloody diarrhea and potentially fatal hemolytic uremic syndrome. We have developed O157:H7-specific bacteriophage ΦV10 to transduce luxCDABE, causing the pathogen to bioluminesce if it is present. We have tested ΦV10-luxagainst diluted O157:H7 at 37°C in beef and romaine lettuce selective enrichments following the protocols from the USDA FSIS and FDA BAM, respectively. Using a luminometer to detect bioluminescence, initial inocula of 10 CFU/g could be detected in beef and 103CFU/g in lettuce in 12 hours, giving a putative positive result even before the real-time PCR step of current protocols.*Carla Rosenfield, PhD candidate in Biological Science, Purdue University 


5:30  Close of Symposium 
Industry, government and academic scientists are encouraged to submit poster titles for this event. One-page abstracts (8 1/2" x 11" with 1-inch margins) must be submitted via e-mail: no later than May 10, 2014 for inclusion in conference documentation. Additional poster submissions will be accepted until May 25, 2014 but may not be included in conference documentation.

DIMENSIONS of the poster boards are: 
4 feet wide by 3 feet high 
(although posterboards could be placed vertically as well and then the dimentsions obviously would be 3' w x 4' h, or 90 x 120cm accordingly). 

Note: If you're submitting a poster, you MUST be registered and paid registration fee plus posterboard reservation fee in advance to ensure that a posterboard is reserved for you. 
Registration fee includes access to the Conference, refreshments, access to posters and exhibit, and all documentation made available to us by speakers. 

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* On-site registration - add US $100 to below amounts 

Commercial Registration: 
Food Safety Symposium Only (June 12, 2014): 
Non-member: US $449 
Member: US $381 
3 DAY - Biodetection, Biosurveillance & Food Safety (June 10-12, 2014): 
Non-member: US $1399.00 
Member: US $1189.15

Academic/Government Registration*:  

Food Safety Symposium Only (June 12, 2014): 
Non-member: US $349 
Member: US $296  
3 DAY - Biodetection, Biosurveillance & Food Safety (June 10-12, 2014): 
Non-member: US $999.00 
Member: US $849.15
Poster Space Reservation fee: 
US $79 (you must be registered for the Conference) 
*The academic/government rate is extended to all participants registering as full time employees of government and universities. To receive the academic/government rate you must not be affiliated with any private organizations either as consultants or owners or part owners of businesses. 

Payment: All payments must be made in U.S. funds drawn on a U.S. bank. Please make check(s) payable to The Knowledge Foundation and attach to the registration form even if you have registered by phone, fax or e-mail. To guarantee your registration, payment must be received prior to the conference. Confirmation of your booking will follow. 

Discount Accommodations and Travel: 
A block of rooms has been allocated at a special reduced rate. Please make your reservations by October 21, 2013 to obtain this rate. When making reservations, please refer to The Knowledge Foundation. Contact The Knowledge Foundation if you require assistance. 

Conference Venue:
Baltimore Harbor Hotel 
(formerly Sheraton Baltimore City Center Hotel) 
101 West Fayette Street
Baltimore, MD  21202

A substitute member of your company may replace your attendance at any time at no charge if you find your schedule prevents you from attending. Please notify us immediately so that materials can be prepared. If you do not wish to substitute your registration, we regret that your cancellation will be subject to a $100 processing fee. To receive a prompt refund, we must receive your cancellation in writing 30 days prior to the conference. Unfortunately cancellations cannot be accepted after that date. In the event that The Knowledge Foundation cancels an event, The Knowledge Foundation cannot resume responsibility for any travel-related costs.