Webcast Agenda - Times are U.S. Pacific Standard TIME
Wednesday, November 12, 2008
10:15 Nanoparticles Based Biosensors for Sensitive Detection of Biomarkers
Yuehe Lin, PhD, Laboratory Fellow, Pacific Northwest National Laboratory
The utilization of functional nanoparticles for biosensing has generated a great deal of interest because they are found to greatly enhance sensitivity of various given methods. Electrochemical biosensors based on nanoparticle labels are very attractive for bioassays, due to their high sensitivity, inherent simplicity, miniaturization, and low cost. In this presentation, we will give an overview on recent development of nanoparticles based biosensors for selective and sensitive detection of protein biomarkers.
10:45 Nanoparticles and Quantum Dots for Biodiagnostic Applications
Arnold Kell, PhD, and Benoit Simard, PhD, Principal Research Officer, Steacie Institute for Molecular Sciences, National Research Council, Canada*
Infectious and chronic diseases and bioterrorism are of tremendous global concern. The National Research Council of Canada is dedicated to developing nanoparticle platforms to aid in the rapid detection of these deadly diseases and security threats. Specifically, this presentation will highlight our efforts to utilize unique surface ligands anchored to magnetic nanoparticles, dye-doped silica nanoparticles and quantum dots to enable the selective labeling, magnetic confinement and optical detection of a variety of potentially harmful bacteria and cells in biological samples.
*In collaboration with: Kui Yu
11:15 DNA-Tile-Array Based Biosensors
Yan Liu, PhD, Assistant Professor, Dept of Chemistry and Biochemistry, Biodesign Institute, Center of Single Molecule Biophysics, Arizona State University
We describe a new concept using combinatorial self-assembly of DNA nanotiles into micrometer-sized two-dimensional arrays that carry nucleic acid probes and barcoded fluorescent dyes to achieve multiplexed detection. The specificity and sensitivity of the arrays by detecting multiple DNA sequences and aptamer binding molecules were demonstrated. Accurate control of the interprobe distances and solution-based binding reactions ensures fast target binding kinetics. The detection sensitivity can be future improved using hybridization chain reaction (HCR) as a signal amplifier.
12:15 Electric Field Assisted Nano-Assembly and Detection on Micoarrays
Dalibor Hodko, PhD, Director, Advanced Technology, Nanogen, Inc.
Principles and examples of electronic microarray applications for electric-field assisted (nano-)assembly of molecules and components for sensors and detection of pathogens will be summarized. Electric field and hydrodinamically enhanced biochemical reactions will be demonstrated in the array format such as rolling circle amplification for isothermal on-chip amplification with sensitivity approaching few molecules. Attempts toward miniaturization of the electronic microarray platform will be summarized with intended point-of-care applications.
12:45 Point-Of-Care Detection of Staphylococcal Enterotoxins
Hugh Bruck, PhD, Associate Professor of Mechanical Engineering, University of Maryland
Staphylococcal enterotoxins (SEs) are amajor cause of food-borne diseases. Traditionally, SEs assayed immunologically with ELISA. Carbon nanotubes’ (CNT) uniquemechanical and electronic properties combined with a large specific surface areamake them attractive for biosensing.We applied CNT to increase the sensitivity of a simple and portable point-of-care immunosensor based on the detection of Enhanced chemiluminescence (ECL) by a cooled Charge- Coupled Device (CCD) detector. This combination of ECL, CNT and CCD technologies is used to improve the detection of Staphylococcal Enterotoxin B (SEB) in food. Anti-SEB primary antibodies were immobilized onto the CNT surface and the antibody-nanotube mixture was immobilized onto a polycarbonate surface. SEB was then detected by a sandwich-type ELISA assay on the CNTpolycarbonate surface with an ECL assay. SEB in buffer, soymilk, apple juice, andmeat baby food was assayed with a limit of SEB with CCD detector is 0.01 ng/mL similar to the detection limit of a fluorometric detector, which is farmore sensitive than the conventional ELISA detection limit of ~1 ng/ml. Our simple, versatile and inexpensive CCD based Point-of-care detector combined with the CNT-ECL immunosensormethod can be used to simplify and increase sensitivity formany other types of diagnostics and detection assays.
2:30 Simultaneous Multiplexed Single-Molecule Detection of DNA, RNA, and Proteins
Michael D. Berg, PhD, Chief Scientific Officer, Attometrics, Inc.
We report the construction of a novel biosensing nanodevice to detect single molecules of target DNA, RNA, or protein on the same platform at the same time. The combination of reliable specificity, high sensitivity, and low cost should make this technology a viable option for widespread clinical use. Early diagnosis of diseases such as cancer can have a profound impact on a patient’s prognosis. The diagnoses are dependent on rapid, sensitive detection of specific protein and/or nucleic acid biomarkers. Rapid, sensitive detection and identification of pathogens is also critically important to minimize the transfer and spread of disease as well as to devise and evaluate effective treatment strategies.
3:00 TessArray® Resequencing Pathogen Microarrays (RPM): Engineering Networks of Nanoscale Information Transducers for Simultaneous, Definitive and Differential Detection and Identification of Multiple Pathogens
Clark Tibbetts, PhD, Executive Vice President and CTO, Tessarae, LLC*
Consider that the oligonucleotide probes, as typically used to amplify specific target gene sequences, are actually nanoscale information transducers. A single 25-nucleotide probe (8 nm length) represents extraordinary sequence specificity, as only one of 1015 different sequences of the four bases A, G, C and T. The intrinsic differences among probe sequences, that confer such specificity to their transducing hybridization with complementary target sequences, also leads to variation in the avidity of such interactions. This is further complicated by complementary sequence mismatches (as from mutations) that may weakly or strongly perturb the notionally specific interactions of probes and targets. The oligonucleotide probe can thus be considered to be a specific nanoscale information transducer, albeit a relatively noisy one. This is the basis of surprisingly high rates of false negative and false positive results from PCR-based diagnostic tests in real-world applications. Such real-world application example demonstrates the capability of a TessArray RPM assay for rapid and unequivocally accurate detection and identification of any HN subtype of human and/or avian influenza viruses. *In collaboration with: K.Schafer and M.Lorence
3:30 Detection and Subtyping of Avian Influenza Virus and Foot-and-Mouth Disease Virus Using the NanoChip400 Electronic Microarray
Oliver Lung, PhD, Research Scientist, Canadian Food Inspection Agency, Animal Diseases Research Institute, Canada*
Avian influenza virus (AIV) and Foot-and-Mouth Disease virus (FMDV) outbreaks can result in severe economic losses. We developed two electronic microarrays that can subtype all 16 H (hemagglutinin) subtypes of AIV, and all 7 serotypes of FMDV. Probes were also designed against the conserved regions of the AIV matrix (M) gene and FMDV polymerase coding region to detect all AIV and FMDV, respectively. Thirty-two influenza isolates representing all 16 H subtypes, and 23 FMDV isolates representing all 7 serotypes were correctly identified and typed using the two arrays. *In collaboration with: A.Beeston, J.Geddes, K.Burton Hughes, J.Pasick, A.Clavijo, T.Furukawa-Stoffer, W.Mauro, and D.Deregt, Canadian Food Inspection Agency; and D.Hodko, Nanogen
4:30 Chemical and Biosensor Applications Based on Hybrid Nanojunction Arrays
NJ Tao, PhD, Professor of Electrical Engineering; and
Erica Forzani, PhD, Research Assistant Professor, Chem- & Bio- Sensors, Center for Bioelectronics and Biosensors, Biodesign Institute, Arizona State University
5:00 Nanotoxicology: From Emergency Services Response to Environmental Health Considerations
Michelle Cadieux, MBA, HM FRO, CNCP, Researcher, Disaster Services Professional, NanEngineering (NASA)/ Community Safety Programs
Chemical detection equipment is becoming more and more sensitive as the particles are smaller, yet not all chemical sampling equipment is in this range yet. Nano sized particles have different properties than the regular chemicals. There are already studies finding carbon nanotubes have asbestos like qualities and the bioaccumulative effects found in fish brains. Come discuss nanotoxicology, how the industry is changing. Resources on PPE, which MSDS chemical databases track at this level, and a survey of field sampling equipment that work on the nano scale. The Nano Safety Consortium is working with the EPA to have companies voluntarily come forward with safety data.
5:30 End of Live Webcast