June 10-12, 2014 • Baltimore, MD  USA
 
Conveniently Timed and Co-Located With 
 
   
 
 
 
 
 
       
 
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  Summit Overview   
  Biodetection Agenda   
  Sponsoring Opportunities   
  Food Safety Agenda   
  Biosurveillance Agenda   
 
 
 
As technology progresses and innovations evolve, professionals in areas of biosensing, biodetection, bioforensics, biosurveillance, bioinformatics, bionanotechnologies, and sample prep are being challenged to proactively respond to the growing threat of bioterrorism and infectious diseases. Join fellow scientists, industry professionals, physicians and point-of-care practitioners, homeland security experts, public health researchers, first responders, and policymakers from around the globe to define the future of this robust and rapidly expanding field of science and technology.

This international summit will address the following aspects within the framework of each of the 5 conference tracks:

    •    Fundamental research and innovation
    •    Product and business development
    •    Field testing and clinical trials
    •    Financing, mergers and acquisitions
    •    Intellectual property and licensing
    •    Industrial products and manufacturing
    •    Process automation
    •    Marketing and commercialization
    •    Distribution and operations
    •    Regulatory issues and standardization
 
 
 
 
 
 
Media Sponsors and Conference Partners 
 
 
 
  
 
 
 
 
 
 
 
 
Tuesday, June 10, 2014 
 
7:30 Registration with Morning Coffee 
 
8:20 Welcome Remarks from Conference Director 
 
GENE AMPLIFICATON FOR BIOLOGICAL TARGET IDENTIFICATION 
 
8:25 Chairperson's Opening Remarks  
 
8:30 Increased Discrimination of Pathogens and Blood Donor Sample Multiplex Testing with an OpenArray Platform 
Robert Duncan, PhD, Staff Scientist, FDA Center for Biologics Evaluation and Research, U.S. Food and Drug Administration 
Detection of pathogens in blood is required for donor screening and diagnostics. We recently demonstrated effective multiplex screening for 9 pathogens simultaneously with the OpenArray nanofluidic real-time PCR platform. The blood borne pathogen OpenArray platform has been expanded to screen 26 pathogens with discrimination to the species, strain or genotype level. High sensitivity of detection was demonstrated with 92 blood donor specimens.
 
9:00 Treatment Guiding Detection of Burkholderia 
R. Paul Schaudies, PhD, CEO, GenArraytion, Inc. 
 
In collaboration with USAMRIID, thirty isolates each of Burkholderia pseudomallei and mallei were screened using a genotyping microarray. Results were combined with antibiotic sensitivities to generate multiplexed real-time PCR to identify and characterize these organisms.
 
9:30 Conductive DNA Real-Time Sumultaneous Detection of Multiple Targets 
Fred Albert, PhD, President, Bridger Technologies, Inc. 
While progress is being made on the rapid and simultaneous detection of multiple targets, evolutionary improvements to conventional biodetection systems are unlikely to overcome their inherent limitations. A breakthrough technology that enables the simultaneous detection of multiple targets in less than one minute will be presented. This non-PCR, conductive DNA-based technology has been shown to be highly sensitive, highly specific, and deliver accurate results even in the presence of background and contaminants that debilitate or foul other detection systems.
 
10:00 Coffee Break with Exhibit and Poster Viewing 
 
10:30 A Fully Autonomous Biodetection System for Environmental Surveillance 
Mark Burton, Northrop Grumman Corporation 
The Next Generation Automated Detection System (NG-ADS) Biodetector that continuously collects and analyzes air samples to detect and identify biological threat agents will be presented.  The biodetector operates autonomously between routine consumables replenishment, and can be operated 24/7 year round. The detector is designed to use a multiplexed PCR assay to reliably detect threat agents simultaneously with high sensitivity, low limit of detection, and an extremely low false positive rate.  Samples are archived for further analysis if desired.  These systems have recently participated in a field test to demonstrate performance in an operational environment.
 
11:00 Addressing Complex Clinical Problems with Novel Diagnostic Strategies 
Harshini Mukundan, PhD, Research Scientist, Los Alamos National Laboratory 
We will discuss advanced and integrated biodiagnostic strategies at LANL, spanning from rapid biomarker detection to advanced sequencing based analysis, to understand the circulation, emergence and occurrence of drug resistance in a pediatric population in rural Kenya. In addition, we will also talk about novel detection strategies used for the detection of bacterimia, including active tuberculosis, for the first time in this population.
 
11:30 Enabling Point of Care Test Development for the STD Market 
Joany Jackman, PhD, Investigator, Center for Point of Care Tests for STD, Johns Hopkins University School of Medicine* 
The mission of the Johns Hopkins University Center for Point of Care Tests for Sexually Transmitted Diseases (JHUC) is to provide expertise, guidance and samples to enable the development of the best available test platforms for diagnosis of sexually transmitted infections (STIs). To that end JHUC has conducted focus groups, facilitated meetings and other studies to determine the most important attributes of a successful test for STIs in a variety of point of care settings.  These data and their relevance to the global market for POCT for STIs will be presented.*In collaboration with: M.Jett-Goheen, A.Rompalo, T.Hogan, C.Gaydos
 
12:00pm Sponsored Presentation (Opportunity Available)  
 
12:30 Luncheon Presentation (Sponsorship Opportunity Available) or Lunch on Your Own 
 
NOVEL DETECTION STRATEGIES FOR BIOLOGICAL TARGETS 
 
1:55 Chairperson's Opening Remarks  
 
2:00 Direct Multiplexed Virus Detection 
John H. Connor, PhD, Professor, Dept of Microbiology, Boston University School of Medicine 
We have developed an LED-based virus detection technology that is label-free and multiplexed. This technology allows the identification of viruses that cause hemorrhagic fever without the need for nucleotide isolation and amplification on a rapid time-scale in platform that can be used at the point of care.
 
2:30 Optimization of a Lateral Flow Immunoassay (LFI) for the Rapid Diagnosis of Melioidosis 
David AuCoin, PhD, Associate Professor, Dept Microbiology and Immunology, University of Nevada School of Medicine 
Burkholderia pseudomallei is a soil-dwelling bacterium that is the causative agent of melioidosis.  Laboratory detection of B. pseudomallei is difficult and slow, because of challenges with culturing and a lack of validated diagnostic reagents, but this has been the best approach for diagnosis of melioidosis.  Our goal, therefore, has been to develop a rapid point-of-care immunoassay for the diagnosis of melioidosis.  Our initial efforts have focused on developing a CPS-specific monoclonal antibody (mAb).  The same mAb was used to produce a prototype lateral flow immunoassay (LFI) that is capable of detecting CPS in a variety of patient samples. The CDC is currently testing the LFI against a large panel of B. pseudomallei, related and near neighbor strains.  Results of these tests and plans for further field testing will be reported.
 
3:00 Towards PCR-Free, Visual DNA Detection 
Mahesh Uttamchandani, PhD, Assistant Professor, DSO National Laboratories, National University of Singapore, Singapore 
Novel methods to detect DNA sequence specifically through color change reactions will be described. To transduce molecular recognition events into visual readouts, we have engineered assays which have exploited split DNAzymes and gold nanoparticles. The G-quadruplex DNAzymes were successfully applied to the detection of Salmonella and Mycobacterium DNA, as well as in genotyping a single base difference from within human genomic DNA samples.  An integrated workflow was capable of detecting DNA samples through a color change within just 5-15 minutes. The gold nanoparticle method offered much greater sensitivity, lowering the limit of detection visually, without the need for PCR amplification.
 
3:30 Refreshment Break with Exhibit and Poster Viewing 
 
4:00 Magneto-Hydrodynamic Focusing for Point of Care Applications 
Christian Reis, Group Lead - Biotechnology Processes, Fraunhofer IPA, Germany 
Magnetic bead handling is a common tool in on-chip biodetection systems and research is improving fast.  A technology for detecting the load of a magnetic particle by forcing the particle to describe certain trajectories with switching magnets will be presented. This allows us to net-focus magnetic beads in a hydrodynamic system and to provide quantitative insight for the number of molecules bound to the particle surface.
 
4:30 Bioaerosol Standoff Detection Using Lidar Technology Allowing Cloud Mapping and Spectrometric LIF Classification 
Sylvie Buteau, PhD, Scientist, Defence Research and Development Canada, Canada* 
A standoff sensor called BioSense was developed to demonstrate the capacity to map, track and classify bioaerosol clouds from a distant range and over wide area.  The concept of the system is based on a two steps dynamic surveillance: 1) cloud detection using an infrared (IR) scanning cloud mapper and 2) cloud classification based on a staring UV Laser Induced Fluorescence (LIF) interrogation. The main challenge is classification, which relies on a spectrally resolved UV LIF signature library. The system showed good performances even prior to further optimization. *In collaboration with: J.-R.Simard, G.Roy, P.Lahaie

5:00 iTIRF – Cell Phone Based Biosensor for Molecular Diagnostics 
Alexander N. Asanov, PhD, President and CSO, TIRF Labs, Inc 
A novel molecular diagnostics technology based on Total Internal Reflection Fluorescence, termed – iTIRF, will be presented.   iTIRF is capable of simultaneously detecting proteins, nucleic acids, and metabolite biomarkers.  iTIRF microarrays employ silk fibroin, which allows for much greater immobilization of reagents and a resulting signal that is a thousand-fold greater than that with classical TIRF.  Additional advantages of the biosensor, and plans for further development, will also be described.
 
5:30 Genomic-Based Approach for Tracking and Discriminating Pathogens 
Willy Valdivia-Granda, PhD, CEO, Orion Integrated Biosciences Inc. 
The microbiome of an animal contains approximately 10 times the number bacterial cells than host cells and around 150 times more genes.  Using a library of motif fingerprints and genomic signatures for pathogens of biodefense and agrodefense relevance, we performed an extensive survey of the metagenomic samples of humans and domestic animals.  We have used our motif fingerprint scanning technology to perform inclusion/exclusion bioforensic and attribution analysis.  The implications of our work in biosurveillance and standardized nucleic acid- or antibody-based detection system development will be discussed.
 
 
6:00-7:00 Welcome Reception in the Exhibit Hall with Poster Viewing 
 
 
 
 
Wednesday, June 11, 2014 
 
7:30 Morning Coffee 
 
CHEMICAL & TOXIN DETECTION 
 
8:55 Chairperson's Opening Remarks 
 
9:00 Automatic Online Real-Time Detection of Microcystin-LR Based on Optical Biosensing System 
Feng Long, PhD, Research Scientist, School of Environment, Tsinghua University, PR China 
To minimize the health risks to the public, cyanotoxin detection methods that are rapid, sensitive, real time, and high frequency must be established.  An novel automated optical biosensing system (AOBS) was developed for the rapid detection of microcystin-LR (MC-LR). Results using an indirect competitive detection mode will be presented.   The quantification of MCLR ranges from 0.2 to 4 µg/L, with a detection limit determined as 0.09 µg/L.
 
9:30 Microcantilever Enabled Biodetection 
Rick Venedam, PhD, Senior Scientist, National Security Technologies, LLC 
Embedded piezoresistive microcantilever (EPM) sensors have been used in the detection of a variety of analyte species.  EPM sensors utilize a tiny piezoresistive microcantilever partially embedded into a sensing material to produce a sensing element that is compact, simple, resistant to movement and shock, and suitable for remote sensing applications. In this project  we used sensing materials consisting of an immobilizing polymer functionalized with either target enzymes or antibodies to detect two biological agents, Bacillus subtilis and Diisopropyl fluorophosphates, a simulant for organophosphate nerve agents.  Sensing results are presented for both types of EPM sensors.

10:00 A Portable Biochip Based Platform for Detection of Biothreat Agents
Christopher Pöhlmann, Ph.D., Application CBRNE, Bruker Daltonik GmbH

Natural outbreaks and the willful use of highly pathogenic organisms or biological toxins for acts of terror have had tremendous impact on human populations. Currently, the most widely used test to detect the presence of toxins is ELISA, whereas rapid detection of various microorganisms is done by PCR. However, these techniques cannot be performed by untrained personnel in the field and they are time-consuming. Electrochemical transduction of binding events is an alternative for commonly used optical detection. Remarkable advantages of electrochemical biosensing are the high sensitivity, simple operation, and the possibility of being used in portable instruments for on-site testing. Here, we present the electrochemical detection platform pTDTM for universal identification of five biothreat agents including inherent positive and negative controls.
 

 
 
10:30 Coffee Break with Exhibit and Poster Viewing 
 
11:00 Pre-Analytical Complex Sample Preparation Method to Highly Improve Ultra-Sensitive Detection of (Re-) Emerging Pathogens for Accurate Diagnostics
Francisco Veas, Ph.D., Research Professor, Institut de Recherche Pour
 le Développement, France

Ultrasensitive, rapid and reliable diagnostic of infectious agents from complex samples is critical to adopt, the faster the most adapted individual and health countermeasures. Excellent current and innovative methods for pathogens detection encounter difficulties to establish an accurate diagnostic when a rapid reactivity is needed. To adopt an appropriated strategy to reach ultrasensitive diagnostic unmet needs, we have developed a highly performing, fast, simple technology to concentrate and cleanse pathogens to prepare samples for ultrasensitive diagnostics. Our technology is based upon the capacity of β2-glycoprotein I (β2GpI) or ApoH, a human scavenger acute phase protein, able to capture, with a high affinity, a very large spectrum of pernicious microorganisms including Gram + or Gram - bacteria, enveloped or non enveloped DNA or RNA viruses, fungi, and parasites. Here we will show a variety of examples of ultrasensitive detection of pathogens from complex biological samples (human or animal tissues, blood, plasma urine, feces, etc) pretreated with supports coated with synthetic ApoH-derivatives.
 
 
11:30 Point of Care Diagnostic Analysis of Samples in Complex BackgroundsBrad Wright, PhD, Vice President, Bridger Technologies, Inc.
While PCR-based systems seem to have the greatest foothold in point-of-care diagnostic analysis, they also suffer significant limitations with respect to accurate results in complex backgrounds.  This presentation focuses on a breakthrough technology that enables the detection of targets in less than one minute.  This conductive DNA-based technology is extremely simple to operate and has been shown to be highly sensitive, highly specific, and deliver accurate results even in the presence of background and contaminants that debilitate many other systems. 
 
 
 
12:00pm Mid-Infrared QC Lasers for Molecular Recognition 
Mariano Troccoli, PhD, Director of Product Development, AdTech Optics, Inc. 
Recent results with high performance mid-infrared quantum cascade lasers both for high power and single-mode operation will be presented.  In addition, their applications to molecular recognition will be described and results on multi-wavelength detection of important chemical compounds in a single multi-laser system are detailed.
 
12:30 Luncheon Presentation (Sponsorship Opportunity Available) or Lunch on Your Own 
 
1:55 Chairperson's Opening Remarks 
 
2:00 Host-Response Based Biodetection by Immunosignatures 
Stephen Albert Johnston, PhD, Co-Director, Center for Innovations in Medicine, Professor, School of Life Sciences, Biodesign Institute, Arizona State University 
Most of biodetection efforts have focused on sensing the pathogen. This has serious basic and practical limitations. A simple technology based on immunosignatues, for detecting host changes in response to pathogens, will be presented.  It is very sensitive and inexpensive.  It is commercializable and importantly would enable new levels of biosecurity as a by product of standard clinical practice.
 
TOOLS FOR DETECTION - MICROFLUIDICS & ENZYMES 
 
2:30 Tool Box of Engineered Microfluidic Components Shortens Development Time and Reduce Risk 
Leanna Levine, PhD, CEO, Aline, Inc. 
A toolbox of engineered microfluidic components, including metering channels, valves, vents, pumps, and de-bubbling, can be engineered into any number of desired footprints.  Optimized actuation inputs and protocols, and design specifications ensure well-characterized and repeatable performance.  Through choice of materials and design constraints, we demonstrate data on the repeatable performance of a device that meters, mixes, debubbles and dispenses. Data is presented on component reproducibility and scalable production.

3:00 SoundStream – A Microfluidic-Based Assay Platform for Rapid Portable Diagnostics 
Arlene Doria, PhD, CEO, DEFINEQA Inc. 
An innovative microfluidic technology known as SoundStream, will be described.  The field of microfluidics is plagued with challenges in integration, fluid control, and limited sample preparation strategies.  SoundStream employs the use of oscillating microbubbles to perform multiple assay steps including pumping, mixing, bead assay detection, plasma/serum separation, cell lysis, and particle size separation.  The technology is easy to integrate with bioassay detection methods.  It reduces the complexity of the microchip design and is scalable. Finally, the platform can be powered by simple batteries for rapid portable diagnostics.
 
3:30 Refreshment Break with Exhibit and Poster Viewing 
 
4:00 Application of a Recombinant Topoisomerase for the Specific Enrichment of Prokaryotic DNA 
Natalia Sandetskaya, PhD, Fraunhofer Institute for Cell Therapy and Immunology IZI, Germany 
The development and application of the novel molecular tool for the targeted enrichment of prokaryotic DNA in complex samples will be presented.  The DNA binding subunit of the bacterial topoisomerase II, gyrase, was expressed, purified and immobilized on magnetic particles. Results showing specific affinity towards bacterial DNA in the samples with high background of eukaryotic DNA will be described. This method is a promising approach for the preparation of such type of the samples, for example, in molecular diagnostics of sepsis.
 
4:30 A Novel Thermostable Viral DNA Polymerase Facilitates Point of Care Molecular Detection of DNA and RNA Targets 
David Mead, PhD, CEO, Lucigen Corp 
Point of care (POC) molecular detection of pathogens requires improvements in enzymes, formulation and stability. OmniAmp enzyme is a novel isothermal amplification polymerase for loop-mediated amplification (LAMP) amplification of RNA or DNA.  The unique inherent reverse transcriptase activity of the enzyme allows single enzyme detection of RNA targets.  OmniAmp can be formulated dry for ambient storage and transport. Detection of amplification products can be accomplished using multiple methods.
 
5:00 End of the Biodetection Technologies Conference 
 
 - 
 
Thursday, June 12, 2014 

8:00 Registration with Morning Coffee 
 
8:45 Chairperson’s Welcome and Opening Remarks
John Doesburg, International Security & Analysis Programs, Oak Ridge National Laboratory (ORNL) 
 
9:00 Biosurveillance Portal: Situational Awareness for the Department of Defense
C. Nicole Rosenzweig, PhD, Research Biologist, Edgewood Chemical Biological Center, U.S. Army RDECOM Laboratory
The Joint Program Executive Office (JPEO) is the Joint Services manager for acquisition and fielding information management systems for the Warfighter.  Currently, JPEO is executing an Advanced Technology Demonstration (ATD) which includes an informatics effort: the Biosurveillance Portal.  To support the situational awareness needs of the Warfighter, development of a common informatics architecture is the first order of business.  To this end, the Operational Release of the Biosurveillance Portal is October, 2014.  With this defined software stack, the portal will support seamless communication and data sharing in the US Pacific Command.  The user base will be expanded to other US Commands in FY15, with functionality expansion planned for each release.
 
9:30 National Biosurveillance Integration Center Enabling Shared Situational Awareness of Acute Biological Events Through Rapid Identification, Characterization, Localization and Tracking
Steve Bennett, Ph.D. Director, National Biosurveillance Integration Center, Office of Health of Affairs, U.S. Department of Homeland Security 

10:00 Scalable “Big Data” Machine Learning for Bio-surveillance
Arvind Ramanathan, Ph.D., Computational Biologist, Oak Ridge National Laboratory and Laura Pullum, Ph.D., Research Scientist, Oak Ridge National Laboratory

As the number of data sources for public health surveillance continues to grow both in volume and variety, there is a need to develop data-driven machine learning tools that can automate discovery and aid decision makers in obtaining quantifiable insights on emerging disease spread phenomena. In this talk, we present an overview of scalable machine learning tools that we have been developing as part of advancing this mission. In particular, our machine learning tools can automatically (1) detect multi-scale spatial and temporal break-out patterns of disease occurrence, (2) quantify multi-modal co-occurrence disease patterns to identify local- and national-level “hotspots” and (3) predict how patterns of co-occurrence correspond to ‘intervention’ strategies. We will present vignettes of our toolset in the context of analyzing both social media and electronic health transactional data and demonstrate its applicability in a retrospective analysis of the 2009-2010 H1N1 flu season. 

10:30 Coffee with Exhibit & Poster Viewing 
 
11:00 U.S. Government Perspective on Medical Preparedness for the National Strategy for Biosurveillance
Susan Coller Monarez, Ph.D., Director for Medical Preparedness Policy, National Security Council Staff, Executive Office of the President of the United States 
 
11:30 Common Platforms for Diagnostic and Environmental Assays Provide Comprehensive Situational Awareness for Diverse Missions
Amy L. Altman, Ph.D., 
Vice President, Biodefense, Luminex Corporation
Ensuring the safety of our food supply is critical as unsafe food accounts for millions of deaths and cases of disease annually. Our ability to quickly detect and characterize an outbreak of a food-borne illness, whether due to a bioterrorist event or inadvertent food contamination, is critical for saving lives and minimizing the economic impact of such an incident. Foodborne illness is a growing public health problem and sensitive and accurate detection of the causative agent presents unique instrumentation and assay challenges. Determining the source of the outbreak also presents challenges as the first sign of a potential food borne outbreak is typically a surge of patients seeking medical care for gastrointestinal symptoms. To provide more comprehensive situational awareness, efforts at Luminex include developing both a more comprehensive assay menu and a suite of instruments to address varying mission needs from high throughput laboratories to field assessment. Open-architecture xMAP® technology allows simultaneous detection of bacterial, viral and toxin agents, in a highly flexible, multiplexed architecture capable of protein and nucleic acid assay formats. We will discuss how diagnostics and environmental detection assays can work synergistically to provide timely and accurate answers.
 
12:00 Lunch on Your Own 
 
1:30 DTRA’s  Biosurveillance Ecosystem
Ronald K. Hann Jr., Ph.D, Director CB Technologies, Defense Threat Reduction Agency/Joint Science and Technology Office (DTRA/JSTO) 
 
2:00 Integrative Biosurveillance
Harshini Mukundan, PhD, Principal Investigator, Chemistry Division, Los Alamos National Laboratory
Biosurveillance requires the integration of complex data from variant sources. Herein we present some of the critical technological tools that can advance that ultimate mission: from advanced diagnostics for pathogen characterization to modeling algorithms. Specifically, we will outline the advancements made and lessons learned in applying these tools to a real world population in rural Kenya. This work is a collaboration between the Los Alamos National Laboratory and the University of New Mexico (center for global health).    

2:30 Development of a JUPITR/GBTI Exemplar Training Laboratory for Evaluation and Optimization of Capability: A new approach.
David L Hirschberg, Assistant Professor of Clinical Pathology & Chief Technology Officer, Columbia University
A Joint USFK Portal and Integrated Threat Reduction (JUPITR) Advanced TEchnology Demonstration (ATD) and Global Biosurveillance Technology Initiative (GBTI) Exemplar laboratory located at Edgewood Chemical Biological Center (ECBC) will serve as a resource for many stakeholders and fulfill several programmatic roles. A testbed for component, reagent, and bioinformatics upgrades, the lab will optimize capabilities in biosurveillance technologies and function as a operation training laboratory for the US Army Public Health Command. The laboratory will also serve as a technology demonstration site for US Army Test and Evaluation Command assessment of JUPITR ATD capabilities ahead of operational demonstration by US Forces Korea (USFK) in 2015.
 
3:00 PANEL DISCUSSION: R&D Objectives of The National Strategy for Biosurveillance
Moderator: 
John Doesburg, International Security & Analysis Programs, Oak Ridge National Laboratory (ORNL)
 
 
Panelists: 
C. Nicole Rosenzweig, PhD, Research Biologist, Edgewood Chemical Biological Center, U.S. Army RDECOM Laboratory
Harshini Mukundan, PhD, Principal Investigator, Chemistry Division, Los Alamos National Laboratory
 
Matthew Lesho, PhD, Director, Government Business Development, Luminex Corporation
 
David L Hirschberg, Assistant Professor of Clinical Pathology & Chief Technology Officer, Columbia University 
 
 
 
 
 
3:30 End of Symposium 
 
 
 
Thursday, June 12, 2014 
 
2:00 Registration with Exhibit & Poster Viewing 
 
2:20 Chairperson’s Welcome and Opening Remarks
Byron Brehm-Stecher, Ph.D., Associate Professor, Iowa State University 

2:30 Light Scattering and Optical Sensors for High Throughput Screening of Bacterial Pathogens
Arun Bhunia, BVSc, Ph.D., Professor of Molecular Food Microbiology, Center for Food Safety Engineering, Dept. of Food Science and Dept. of Comparative Pathobiology, Purdue University
 

Rapid high throughput pathogen screening tools are crucial in improving safety of our food supply. Current pathogen detection trend emphasizes the application of single platform for detection of multiple pathogens/toxins in a cost effective manner. Our research team is currently developing high throughput nano/biosensor-based screening tools such as fiber optic, cell-based and light scattering for detection of multi-pathogens and toxins from food. Cell-based sensor determines virulence potential and is suitable for confirmation of pathogens or toxins present in a sample while light scattering sensor is a label-free method for real-time detection and identification of bacterial colonies on agar plates. While light scattering sensor can detect and identify bacterial colonies on plate without the use of any labeling reagents. It can also be used for bacterial community analysis on products and for process verification, and hygiene monitoring. Limit of detection, specificity, automation and application of each sensor with various sample matrices will be discussed. 
 
 
3:00 Flow Cytometry for Rapid Detection of Pathogens in Complex Matrices
Byron Brehm-Stecher, Ph.D., Associate Professor, Iowa State University
Flow cytometry (FCM) is a rapid method for analysis of cells in liquid suspension. Although FCM was originally developed for measurement of mammalian cells, it has found increasing use in microbiology over the past two decades. FCM allows collection of data at the single cell level on cell physiology, number, viability, genetic identity and other parameters. Depending on the staining technique used, FCM can also be a very robust means for analysis of complex samples, enabling operators to differentiate between cells of interest and high backgrounds of particulate matter or non-target microflora. This talk will provide an overview of FCM and its applications in food microbiology for the detection and characterization of foodborne microorganisms.
 
 
3:30 Parallel Capillary Electrophoresis for High-Throughput Detection and Characterization of Pathogens
Pierre Vareneau, Ph.D., Chief Technology Officer, Advanced Analytical Technologies, Inc.
Capillary electrophoresis (CE) has long been a workhorse technology in analytical chemistry labs. CE provides rapid and efficient separations of biologically relevant molecules or compounds, can be used to analyze small sample volumes and typically requires minimal sample preparation. A key advantage of CE is its superior ability to separate complex pools of nucleic acid fragments that present distinct challenges to the gel-based electrophoresis systems that are most commonly used for DNA analysis. This talk will focus on the use of high-throughput parallel CE for nucleic acid-based analysis of foodborne pathogens, contrasting this approach from traditional analyses, including gel-based systems. Additional applications of parallel CE, including next-generation sequencing and related uses will be discussed. 
 
 
4:00 Refreshments with Exhibit & Poster Viewing 

4:30 Antimicrobial Peptide Sensor Arrays for Endotoxin Detection and Discrimination: Towards Microbial Forensics Applications
Joshua R. Uzarski, PhD, Research Scientist, Battelle Memorial Institute, contractor for US Army Natick Soldier Research, Development, and Engineering Center

Lipopolysaccharides, also called endotoxin, shed from Gram negative bacterial pathogens, are the cause of septic shock syndrome and are a pressing issue in sterile environments including health and food preparation facilities. Current detection methods are insufficient, being both temporally and monetarily expensive. We have developed a method using a small array of antimicrobial peptides that detect and discriminate LPS molecules from different bacterial species, strains, and also from different original growth conditions. The latter example might help identify the source of an endotoxin or pathogen outbreak, augmenting the field of microbial forensics.
 

5:00 Bioluminescence-transducing phage for detection/identification of E. coli O157:H7 
Bruce Applegate, Associate Professor in Biological Sciences and Food Science, Purdue University*

Shiga-toxigenic strains of E. coli, especially E. coli O157:H7, are most frequently associated with beef and produce, and they can cause serious disease such as severe bloody diarrhea and potentially fatal hemolytic uremic syndrome. We have developed O157:H7-specific bacteriophage ΦV10 to transduce luxCDABE, causing the pathogen to bioluminesce if it is present. We have tested ΦV10-luxagainst diluted O157:H7 at 37°C in beef and romaine lettuce selective enrichments following the protocols from the USDA FSIS and FDA BAM, respectively. Using a luminometer to detect bioluminescence, initial inocula of 10 CFU/g could be detected in beef and 103CFU/g in lettuce in 12 hours, giving a putative positive result even before the real-time PCR step of current protocols.*Carla Rosenfield, PhD candidate in Biological Science, Purdue University
 

 

 
 
5:30 Close of Symposium 
 
 
 
 
 
 
 
 
 
Industry, government and academic scientists are encouraged to submit poster titles for this event. One-page abstracts (8 1/2" x 11" with 1-inch margins) must be submitted via e-mail: SUBMIT@knowledgefoundation.com no later than May 8, 2014 for inclusion in conference documentation. Additional poster submissions will be accepted until May 25, 2014 but may not be included in conference documentation. 
 
DIMENSIONS of the poster boards are: 
4 feet wide by 3 feet high 
(although posterboards could be placed vertically as well and then the dimentsions obviously would be 3' w x 4' h, or 90 x 120cm accordingly).
Note: If you're submitting a poster, you MUST be registered and paid registration fee plus posterboard reservation fee in advance to ensure that a posterboard is reserved for you. 
 
 
 
 

Registration fee includes access to the Conference, refreshments, access to posters and exhibit, and all documentation made available to us by speakers. 

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* On-site registration - add US $100 to below amounts 

Commercial Registration: 
 
 
2 DAY - Biodetection Only (June 10-11, 2014): 
Non-member: US $1099.00 
Member: US $934.15 
 
3 DAY - Biodetection, Biosurveillance & Food Safety (June 10-12, 2014): 
Non-member: US $1399.00 
 
Member: US $1189.15
 

Academic/Government Registration*:  

2 DAY - Biodetection Only (June 10-11, 2014): 
Non-member: US $799.00 
Member: US $679.15  
 
3 DAY - Biodetection, Biosurveillance & Food Safety (June 10-12, 2014): 
Non-member: US $999.00 
 
Member: US $849.15
 
 
Poster Space Reservation fee: 
US $79 (you must be registered for the Conference) 
-
 
*The academic/government rate is extended to all participants registering as full time employees of government and universities. To receive the academic/government rate you must not be affiliated with any private organizations either as consultants or owners or part owners of businesses. 
 
 

Payment: All payments must be made in U.S. funds drawn on a U.S. bank. Please make check(s) payable to The Knowledge Foundation and attach to the registration form even if you have registered by phone, fax or e-mail. To guarantee your registration, payment must be received prior to the conference. Confirmation of your booking will follow. 

Discount Accommodations and Travel: 
A block of rooms has been allocated at a special reduced rate. Please make your reservations by May 10, 2014 to obtain this rate. When making reservations, please refer to The Knowledge Foundation. Contact The Knowledge Foundation if you require assistance.
 

Conference Venue:
Baltimore Harbor Hotel 
(formerly Sheraton Baltimore City Center Hotel)
 
101 West Fayette Street
Baltimore, MD  21202
 
 
 
 
 
 
 


Substitutions/Cancellations:
 
A substitute member of your company may replace your attendance at any time at no charge if you find your schedule prevents you from attending. Please notify us immediately so that materials can be prepared. If you do not wish to substitute your registration, we regret that your cancellation will be subject to a $100 processing fee. To receive a prompt refund, we must receive your cancellation in writing 30 days prior to the conference. Unfortunately cancellations cannot be accepted after that date. In the event that The Knowledge Foundation cancels an event, The Knowledge Foundation cannot resume responsibility for any travel-related costs.
 

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