Tuesday, June 10, 2014
7:30 Registration with Morning Coffee
8:20 Welcome Remarks from Conference Director
GENE AMPLIFICATON FOR BIOLOGICAL TARGET IDENTIFICATION
8:25 Chairperson's Opening Remarks
8:30 Increased Discrimination of Pathogens and Blood Donor Sample Multiplex Testing with an OpenArray Platform
Robert Duncan, PhD, Staff Scientist, FDA Center for Biologics Evaluation and Research, U.S. Food and Drug Administration
Detection of pathogens in blood is required for donor screening and diagnostics. We recently demonstrated effective multiplex screening for 9 pathogens simultaneously with the OpenArray nanofluidic real-time PCR platform. The blood borne pathogen OpenArray platform has been expanded to screen 26 pathogens with discrimination to the species, strain or genotype level. High sensitivity of detection was demonstrated with 92 blood donor specimens.
9:00 Treatment Guiding Detection of Burkholderia
R. Paul Schaudies, PhD, CEO, GenArraytion, Inc.
In collaboration with USAMRIID, thirty isolates each of Burkholderia pseudomallei
were screened using a genotyping microarray. Results were combined with antibiotic sensitivities to generate multiplexed real-time PCR to identify and characterize these organisms.
9:30 Conductive DNA Real-Time Simultaneous Detection of Multiple Targets
Fred Albert, PhD, President, Bridger Technologies, Inc.
While progress is being made on the rapid and simultaneous detection of multiple targets, evolutionary improvements to conventional biodetection systems are unlikely to overcome their inherent limitations. A breakthrough technology that enables the simultaneous detection of multiple targets in less than one minute will be presented. This non-PCR, conductive DNA-based technology has been shown to be highly sensitive, highly specific, and deliver accurate results even in the presence of background and contaminants that debilitate or foul other detection systems.
10:00 Coffee Break with Exhibit and Poster Viewing
10:30 A Fully Autonomous Biodetection System for Environmental Surveillance
Mark Burton, Northrop Grumman Corporation
The Next Generation Automated Detection System (NG-ADS) Biodetector that continuously collects and analyzes air samples to detect and identify biological threat agents will be presented. The biodetector operates autonomously between routine consumables replenishment, and can be operated 24/7 year round. The detector is designed to use a multiplexed PCR assay to reliably detect threat agents simultaneously with high sensitivity, low limit of detection, and an extremely low false positive rate. Samples are archived for further analysis if desired. These systems have recently participated in a field test to demonstrate performance in an operational environment.
11:00 Addressing Complex Clinical Problems with Novel Diagnostic Strategies
Harshini Mukundan, PhD, Research Scientist, Los Alamos National Laboratory
We will discuss advanced and integrated biodiagnostic strategies at LANL, spanning from rapid biomarker detection to advanced sequencing based analysis, to understand the circulation, emergence and occurrence of drug resistance in a pediatric population in rural Kenya. In addition, we will also talk about novel detection strategies used for the detection of bacterimia, including active tuberculosis, for the first time in this population.
11:30 Enabling Point of Care Test Development for the STD Market
Joany Jackman, PhD, Investigator, Center for Point of Care Tests for STD, Johns Hopkins University School of Medicine*
The mission of the Johns Hopkins University Center for Point of Care Tests for Sexually Transmitted Diseases (JHUC) is to provide expertise, guidance and samples to enable the development of the best available test platforms for diagnosis of sexually transmitted infections (STIs). To that end JHUC has conducted focus groups, facilitated meetings and other studies to determine the most important attributes of a successful test for STIs in a variety of point of care settings. These data and their relevance to the global market for POCT for STIs will be presented.*In collaboration with: M.Jett-Goheen, A.Rompalo, T.Hogan, C.Gaydos
12:00 pm Sponsored Presentation (Opportunity Available)
12:30 Luncheon Presentation (Sponsorship Opportunity Available) or Lunch on Your Own
NOVEL DETECTION STRATEGIES FOR BIOLOGICAL TARGETS
1:55 Chairperson's Opening Remarks
2:00 Direct Multiplexed Virus Detection
John H. Connor, PhD, Professor, Dept of Microbiology, Boston University School of Medicine
We have developed an LED-based virus detection technology that is label-free and multiplexed. This technology allows the identification of viruses that cause hemorrhagic fever without the need for nucleotide isolation and amplification on a rapid time-scale in platform that can be used at the point of care.
2:30 Optimization of a Lateral Flow Immunoassay (LFI) for the Rapid Diagnosis of Melioidosis
David AuCoin, PhD, Associate Professor, Dept Microbiology and Immunology, University of Nevada School of Medicine
Burkholderia pseudomallei is a soil-dwelling bacterium that is the causative agent of melioidosis. Laboratory detection of B. pseudomallei is difficult and slow, because of challenges with culturing and a lack of validated diagnostic reagents, but this has been the best approach for diagnosis of melioidosis. Our goal, therefore, has been to develop a rapid point-of-care immunoassay for the diagnosis of melioidosis. Our initial efforts have focused on developing a CPS-specific monoclonal antibody (mAb). The same mAb was used to produce a prototype lateral flow immunoassay (LFI) that is capable of detecting CPS in a variety of patient samples. The CDC is currently testing the LFI against a large panel of B. pseudomallei, related and near neighbor strains. Results of these tests and plans for further field testing will be reported.
3:00 Towards PCR-Free, Visual DNA Detection
Mahesh Uttamchandani, PhD, Assistant Professor, DSO National Laboratories, National University of Singapore, Singapore
Novel methods to detect DNA sequence specifically through color change reactions will be described. To transduce molecular recognition events into visual readouts, we have engineered assays which have exploited split DNAzymes and gold nanoparticles. The G-quadruplex DNAzymes were successfully applied to the detection of Salmonella and Mycobacterium DNA, as well as in genotyping a single base difference from within human genomic DNA samples. An integrated workflow was capable of detecting DNA samples through a color change within just 5-15 minutes. The gold nanoparticle method offered much greater sensitivity, lowering the limit of detection visually, without the need for PCR amplification.
3:30 Refreshment Break with Exhibit and Poster Viewing
4:00 Magneto-Hydrodynamic Focusing for Point of Care Applications
Christian Reis, Group Lead - Biotechnology Processes, Fraunhofer IPA, Germany
Magnetic bead handling is a common tool in on-chip biodetection systems and research is improving fast. A technology for detecting the load of a magnetic particle by forcing the particle to describe certain trajectories with switching magnets will be presented. This allows us to net-focus magnetic beads in a hydrodynamic system and to provide quantitative insight for the number of molecules bound to the particle surface.
4:30 Bioaerosol Standoff Detection Using Lidar Technology Allowing Cloud Mapping and Spectrometric LIF Classification
Sylvie Buteau, PhD, Scientist, Defence Research and Development Canada, Canada*
A standoff sensor called BioSense was developed to demonstrate the capacity to map, track and classify bioaerosol clouds from a distant range and over wide area. The concept of the system is based on a two steps dynamic surveillance: 1) cloud detection using an infrared (IR) scanning cloud mapper and 2) cloud classification based on a staring UV Laser Induced Fluorescence (LIF) interrogation. The main challenge is classification, which relies on a spectrally resolved UV LIF signature library. The system showed good performances even prior to further optimization. *In collaboration with: J.-R.Simard, G.Roy, P.Lahaie
5:00 iTIRF – Cell Phone Based Biosensor for Molecular Diagnostics
Alexander N. Asanov, PhD, President and CSO, TIRF Labs, Inc
A novel molecular diagnostics technology based on Total Internal Reflection Fluorescence, termed – iTIRF, will be presented. iTIRF is capable of simultaneously detecting proteins, nucleic acids, and metabolite biomarkers. iTIRF microarrays employ silk fibroin, which allows for much greater immobilization of reagents and a resulting signal that is a thousand-fold greater than that with classical TIRF. Additional advantages of the biosensor, and plans for further development, will also be described.
5:30 Genomic-Based Approach for Tracking and Discriminating Pathogens
Willy Valdivia-Granda, PhD, CEO, Orion Integrated Biosciences Inc.
The microbiome of an animal contains approximately 10 times the number bacterial cells than host cells and around 150 times more genes. Using a library of motif fingerprints and genomic signatures for pathogens of biodefense and agrodefense relevance, we performed an extensive survey of the metagenomic samples of humans and domestic animals. We have used our motif fingerprint scanning technology to perform inclusion/exclusion bioforensic and attribution analysis. The implications of our work in biosurveillance and standardized nucleic acid- or antibody-based detection system development will be discussed.
6:00 -7:00 Welcome Reception in the Exhibit Hall with Poster Viewing
Wednesday, June 11, 2014
7:30 Morning Coffee
CHEMICAL & TOXIN DETECTION
8:55 Chairperson's Opening Remarks
9:00 Automatic Online Real-Time Detection of Microcystin-LR Based on Optical Biosensing System
Feng Long, PhD, Research Scientist, School of Environment, Tsinghua University, PR China
To minimize the health risks to the public, cyanotoxin detection methods that are rapid, sensitive, real time, and high frequency must be established. An novel automated optical biosensing system (AOBS) was developed for the rapid detection of microcystin-LR (MC-LR). Results using an indirect competitive detection mode will be presented. The quantification of MCLR ranges from 0.2 to 4 µg/L, with a detection limit determined as 0.09 µg/L.
9:30 Microcantilever Enabled Biodetection
Rick Venedam, PhD, Senior Scientist, National Security Technologies, LLC
Embedded piezoresistive microcantilever (EPM) sensors have been used in the detection of a variety of analyte species. EPM sensors utilize a tiny piezoresistive microcantilever partially embedded into a sensing material to produce a sensing element that is compact, simple, resistant to movement and shock, and suitable for remote sensing applications. In this project we used sensing materials consisting of an immobilizing polymer functionalized with either target enzymes or antibodies to detect two biological agents, Bacillus subtilis and Diisopropyl fluorophosphates, a simulant for organophosphate nerve agents. Sensing results are presented for both types of EPM sensors.
10:00 A Portable Biochip Based Platform for Detection of Biothreat Agents
Christopher Pöhlmann, Ph.D., Application CBRNE, Bruker Daltonik GmbH
Natural outbreaks and the willful use of highly pathogenic organisms or biological toxins for acts of terror have had tremendous impact on human populations. Currently, the most widely used test to detect the presence of toxins is ELISA, whereas rapid detection of various microorganisms is done by PCR. However, these techniques cannot be performed by untrained personnel in the field and they are time-consuming. Electrochemical transduction of binding events is an alternative for commonly used optical detection. Remarkable advantages of electrochemical biosensing are the high sensitivity, simple operation, and the possibility of being used in portable instruments for on-site testing. Here, we present the electrochemical detection platform pTDTM for universal identification of five biothreat agents including inherent positive and negative controls.
10:30 Coffee Break with Exhibit and Poster Viewing
11:00 Pre-Analytical Complex Sample Preparation Method to Highly Improve Ultra-Sensitive Detection of (Re-) Emerging Pathogens for Accurate Diagnostics
Francisco Veas, Ph.D., Research Professor, Institut de Recherche Pour le Développement, France
Ultrasensitive, rapid and reliable diagnostic of infectious agents from complex samples is critical to adopt, the faster the most adapted individual and health countermeasures. Excellent current and innovative methods for pathogens detection encounter difficulties to establish an accurate diagnostic when a rapid reactivity is needed. To adopt an appropriated strategy to reach ultrasensitive diagnostic unmet needs, we have developed a highly performing, fast, simple technology to concentrate and cleanse pathogens to prepare samples for ultrasensitive diagnostics. Our technology is based upon the capacity of Î²2-glycoprotein I (Î²2GpI) or ApoH, a human scavenger acute phase protein, able to capture, with a high affinity, a very large spectrum of pernicious microorganisms including Gram + or Gram - bacteria, enveloped or non enveloped DNA or RNA viruses, fungi, and parasites. Here we will show a variety of examples of ultrasensitive detection of pathogens from complex biological samples (human or animal tissues, blood, plasma urine, feces, etc) pretreated with supports coated with synthetic ApoH-derivatives.
11:30 Point of Care Diagnostic Analysis of Samples in Complex BackgroundsBrad Wright, PhD, Vice President, Bridger Technologies, Inc.
While PCR-based systems seem to have the greatest foothold in point-of-care diagnostic analysis, they also suffer significant limitations with respect to accurate results in complex backgrounds. This presentation focuses on a breakthrough technology that enables the detection of targets in less than one minute. This conductive DNA-based technology is extremely simple to operate and has been shown to be highly sensitive, highly specific, and deliver accurate results even in the presence of background and contaminants that debilitate many other systems.
12:00 pm Mid-Infrared QC Lasers for Molecular Recognition
Mariano Troccoli, PhD, Director of Product Development, AdTech Optics, Inc.
Recent results with high performance mid-infrared quantum cascade lasers both for high power and single-mode operation will be presented. In addition, their applications to molecular recognition will be described and results on multi-wavelength detection of important chemical compounds in a single multi-laser system are detailed.
12:30 Luncheon Presentation (Sponsorship Opportunity Available) or Lunch on Your Own
1:55 Chairperson's Opening Remarks
2:00 Host-Response Based Biodetection by Immunosignatures
Stephen Albert Johnston, PhD, Co-Director, Center for Innovations in Medicine, Professor, School of Life Sciences, Biodesign Institute, Arizona State University
Most of biodetection efforts have focused on sensing the pathogen. This has serious basic and practical limitations. A simple technology based on immunosignatues, for detecting host changes in response to pathogens, will be presented. It is very sensitive and inexpensive. It is commercializable and importantly would enable new levels of biosecurity as a by product of standard clinical practice.
TOOLS FOR DETECTION - MICROFLUIDICS & ENZYMES
2:30 Tool Box of Engineered Microfluidic Components Shortens Development Time and Reduce Risk
Leanna Levine, PhD, CEO, Aline, Inc.
A toolbox of engineered microfluidic components, including metering channels, valves, vents, pumps, and de-bubbling, can be engineered into any number of desired footprints. Optimized actuation inputs and protocols, and design specifications ensure well-characterized and repeatable performance. Through choice of materials and design constraints, we demonstrate data on the repeatable performance of a device that meters, mixes, debubbles and dispenses. Data is presented on component reproducibility and scalable production.
3:00 SoundStream – A Microfluidic-Based Assay Platform for Rapid Portable Diagnostics
Arlene Doria, PhD, CEO, DEFINEQA Inc.
An innovative microfluidic technology known as SoundStream, will be described. The field of microfluidics is plagued with challenges in integration, fluid control, and limited sample preparation strategies. SoundStream employs the use of oscillating microbubbles to perform multiple assay steps including pumping, mixing, bead assay detection, plasma/serum separation, cell lysis, and particle size separation. The technology is easy to integrate with bioassay detection methods. It reduces the complexity of the microchip design and is scalable. Finally, the platform can be powered by simple batteries for rapid portable diagnostics.
3:30 Refreshment Break with Exhibit and Poster Viewing
4:00 Application of a Recombinant Topoisomerase for the Specific Enrichment of Prokaryotic DNA
Natalia Sandetskaya, PhD, Fraunhofer Institute for Cell Therapy and Immunology IZI, Germany
The development and application of the novel molecular tool for the targeted enrichment of prokaryotic DNA in complex samples will be presented. The DNA binding subunit of the bacterial topoisomerase II, gyrase, was expressed, purified and immobilized on magnetic particles. Results showing specific affinity towards bacterial DNA in the samples with high background of eukaryotic DNA will be described. This method is a promising approach for the preparation of such type of the samples, for example, in molecular diagnostics of sepsis.
4:30 A Novel Thermostable Viral DNA Polymerase Facilitates Point of Care Molecular Detection of DNA and RNA Targets
David Mead, PhD, CEO, Lucigen Corp
Point of care (POC) molecular detection of pathogens requires improvements in enzymes, formulation and stability. OmniAmp enzyme is a novel isothermal amplification polymerase for loop-mediated amplification (LAMP) amplification of RNA or DNA. The unique inherent reverse transcriptase activity of the enzyme allows single enzyme detection of RNA targets. OmniAmp can be formulated dry for ambient storage and transport. Detection of amplification products can be accomplished using multiple methods.
5:00 End of Conference